Evaluation of the NucliSENS EasyQ KPC assay for detection of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae.
نویسندگان
چکیده
The NucliSENS EasyQ KPC assay (bioMérieux SA, Marcy l'Etoile, France) was compared with a routinely used phenotypic method for detection of Enterobacteriaceae producing Klebsiella pneumoniae carbapenemase (KPC)-type carbapenemases, using 806 stool samples and rectal swabs. Compared with the phenotypic method, the EasyQ KPC assay had a sensitivity and specificity of 93.3% and 99.0%, respectively, in this setting, with diverse KPC producers not limited to ST258 Klebsiella pneumoniae.
منابع مشابه
Detection of the Klebsiella pneumoniae carbapenemase (KPC) in K. pneumoniae Isolated from the Clinical Samples by the Phenotypic and Genotypic Methods
Background and Objective: The production of carbapenemases especially Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance in isolated Enterobacteriaceae such as K. pneumoniae. The purpose of this study was detected of the carbapenemase producer K. pneumoniae strains with phenotypic and genotypic ...
متن کاملRapid and sensitive detection of blaKPC gene in clinical isolates of Klebsiella pneumoniae by a molecular real-time assay
BACKGROUND The aim of this study was the rapid identification of bla KPC gene in 38 Klebsiella pneumoniae clinical isolates with reduced susceptibility to carbapenems. The modified Hodge Test (MHT) was carried out to phenotypically determine whether resistance to carbapenems was mediated by a carbapenemase. The detection of the bla KPC gene was performed by real-time acid nucleic sequence-based...
متن کاملEvaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of 1 Klebsiella pneumoniae Carbapenemase
*Corresponding author: 17 Teresa Spanu 18 Istituto di Microbiologia 19 Università Cattolica del Sacro Cuore 20 Largo Gemelli, 8 21 00168 – Rome (Italy) 22 Tel: +39 06 30154218; Fax: +39063051152 23 e-mail: [email protected] 24 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00284-12 JCM Accepts, published online ahead of print on ...
متن کاملEvaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of 1 Klebsiella pneumoniae Carbapenemase Gene ( bla
*Corresponding author: 17 Teresa Spanu 18 Istituto di Microbiologia 19 Università Cattolica del Sacro Cuore 20 Largo Gemelli, 8 21 00168 – Rome (Italy) 22 Tel: +39 06 30154218; Fax: +39063051152 23 e-mail: [email protected] 24 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00284-12 JCM Accepts, published online ahead of print on ...
متن کاملRapid detection of Klebsiella pneumoniae carbapenemase genes in enterobacteriaceae directly from blood culture bottles by real-time PCR.
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic in New York City hospitals and have been associated with serious infections globally. A real-time polymerase chain reaction (RT-PCR) assay was developed to detect carbapenem resistance attributable to KPC from blood culture bottles positive for gram-negative bacilli. Culture confirmation of carbapenemase producti...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 51 6 شماره
صفحات -
تاریخ انتشار 2013